Target
Human ANGPTL3
Synonyms
ANGPTL3 | ANGPT5 | ANG-5 | Angiopoietin-5 | Angiopoietin-like 3 | Angiopoietin-related protein 3 | FHBL2 | Angiopoietin 5 | Angiopoietin-like protein 3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Immunogen
Recombinant human ANGPTL3.
Specificity
Recognizes the fibrinogen-like domain (FLD) of human ANGPTL3. Detects a band of ~68kDa and a cleaved band of ~35kDa by Western blot. Does not cross-react with other ANGPTL family proteins.
Applications
- Western blot (1:2000 - 1:5000)
- ELISA (1:1000 - 1:5000)
Usage
ELISA - Direct or indirect: 1:1000-1:5000. Western blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee