Target
Human ANGPTL3
Synonyms
ANGPTL3 | ANGPT5 | ANG-5 | Angiopoietin-5 | Angiopoietin-like 3 | Angiopoietin-related protein 3 | FHBL2 | Angiopoietin 5 | Angiopoietin-like protein 3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Sf-21-derived rhANGPTL3.
Specificity
Recognizes human ANGPTL3. Species cross-reactivity: recombinant murine ANGPTL3-40%. Does not recognize recombinant human ANG1, ANG2 or ANG4.
Applications
- Western blot (1 - 2 µg/ml)
- ELISA
Usage
Suitable for use in Western Blot and ELISA. Western Blot: 1-2 ug/ml Using a colorimetric detection system, the detection limit for rhANGPTL3 is ~25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. ELISA (direct): 0.5-1.0 ug/ml Detects human ANGPTL3. The detection limit for rhANGPTL3 is ~6.5 ng/well.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Reconstitute in sterile PBS.
Storage
Lyophilized and resuspended forms: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee