Human AMPD3

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b,k Monoclonal

AMPD3/901

Unconjugated

Protein G purified

Unmodified. Also available Ready-to-Use, Azide-free, BSA-free.

Recombinant Full-length Human Protein.

Human AMPD3

• IHC
• IHC - Paraffin (2 - 4 µg/ml)
• ICC (1 - 2 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Flow Cytometry (1 - 2 µg/10E6 cells)

It recognizes a protein of ~90kDa, which is identified as Adenosine Monophosphate Deaminase, isoform E (AMPD3). It has 767 amino acids and is assigned an EC 3.5.4.6. It is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. This mAb shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. This mAb is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens. Optimal dilution of the AMPD3 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q01432 NCBI: NM_000480 NP_000471.1