Target
Human AML1 / RUNX1
Synonyms
RUNX1 | AMLCR1 | Aml1 oncogene | AML1 | AML1-EVI-1 | CBF-alpha-2 | CBFA2 | Acute myeloid leukemia 1 | EVI-1 | PEBP2A2 | PEA2-alpha B | AML1-EVI-1 fusion protein | Oncogene AML-1 | PEBP2-alpha B | PEBP2aB
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen
A synthesized peptide derived from human AML1 around the phosphorylation site of Serine 276.
Specificity
Phospho-AML1 (Ser276) Antibody detects endogenous levels of AML1 only when phosphorylated at Serine 276.
Applications
- Immunofluorescence (1:100 - 1:500)
- Western blot (1:500 - 1:2000)
- Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee