Target
Human AMICA / JAML
Synonyms
AMICA1 | AMICA | CREA7-1 | CREA7-4 | Adhesion molecule AMICA | Gm638 | Junctional adhesion molecule | JAML
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived rhAMICA (aa10-265).
Specificity
Recognizes human AMICA.
Applications
- Western blot (1 - 2 µg/ml)
- Flow Cytometry (25 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in Flow Cytometry, Direct ELISA and Western Blot. Flow Cytometry: 25 ug/ml use 10 ul to label 1E6 cells. Direct ELISA: 0.5-1.0 ug/ml detection limit for rhAMICA is ~2 ng/well. Western Blot: 1-2 ug/ml detection limit for rhAMICA is ~25 ng/lane under non-reducing and reducing conditions.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee