Bovine ALPI / Alkaline Phosphatase

Sheep

Bovine (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Lyophilized

Alkaline Phosphatase [Calf intestine].

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.

• Western blot (1:500 - 1:2000)
• ELISA (1:4000 - 1:20000)

ALKALINE PHOSPHATASE antibody has been assayed against 1.0 ug of Alkaline Phosphatase [Calf Intestine] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:1000 to 1:5000 of the reconstitution concentration is suggested for this product.

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl

Reconstitute in 100 µL of deionized water.

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P09923 NCBI: NM_001631 NP_001622.2