Target
Rat Alpha-Fetoprotein
Synonyms
AFP | Alpha-1-fetoprotein | Alpha-fetoprotein | HPAFP | Alpha-fetoglobulin | FETA
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Purification
Ion exchange chromatography
Specificity
Rat AFP / Alpha-Fetoprotein. The reactivity of the antiserum is restricted to alpha-fetoprotein. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against amniotic fluid, rat hepatoma sera and purified alpha-fetoprotein an single characteristic precipitin line is obtained. No reaction is obtained with rat sera of different strains. ross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to mammalian proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in double radial immunodiffusion (Ouchterlony) a precipitation reaction has been observed with mouse amniotic fluid.