Human Alpha-Fetoprotein

Mouse

Human, Monkey, Dog, Pig (tested or 100% immunogen sequence identity)

Mouse, Rat, Bovine, Cat

IgG2a,k Monoclonal

SPM334

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Alpha fetoprotein purified from serum of a hepatoma patient.

Human Alpha-Fetoprotein.

• IHC
• IHC - Paraffin (1 - 2 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This mAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors. The optimal dilution of the Alpha Fetoprotein antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02771 NCBI: NM_001134 NP_001125.1