Human ALOX15 / 15-Lipoxygenase

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity chromatography

Unmodified

Recombinant full length protein of human 15-LO

Recognizes endogenous levels of 15-LO protein.

• IHC (1:50 - 1:200)
• IHC - Paraffin
• Western blot (1:500 - 1:2000)

Western blot analysis of 15-LO expression in COS7 (A), mouse heart (B) whole cell lysates. Immunohistochemical analysis of 15-LO staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16050 NCBI: NM_001140 NP_001131.3