Target
Human ALCAM / CD166
Synonyms
ALCAM | CD166 antigen | MEMD | CD166
Reactivity
Human
(tested or 100% immunogen sequence identity)
Purification
Immunoaffinity purified
Immunogen
NS0-derived recombinant human ALCAM extracellular domain.
Epitope
Extracellular Domain
Specificity
Recognizes human ALCAM. Less than 0.2% cross-reactivity with rmALCAM, rhICAM-2, rhICAM-3, rhE-Selectin and rhP-Selectin is observed.
Usage
Suitable for use in Western Blot. Using plates coated with 100 ul/well of the capture antibody, in combination with 100 ul/well of the detection antibody at 50 ng/ml, an ELISA for sample volumes of 100 ul can be obtained. To arrive at the optimal dose range for this ELISA, set up a two-fold dilution series of the protein standard starting with 4 ng/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 0.2 um sterile-filtered solution in PBS, 50 ug BSA
Reconstitution
Sterile TBS, pH 7.3, 0.1% BSA.
Storage
Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee