Human AKT3

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

9A5.H9.G7

Unconjugated

Protein A affinity chromatography

Unmodified. Also available Unmodified.

Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.

Internal

The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.

• IHC (20 µg/ml)
• Immunofluorescence
• Western blot (1:500 - 1:2000)
• Flow Cytometry
• ELISA (1:2000 - 1:10000)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Anti-AKT3 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kD in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9Y243 NCBI: NM_005465 NP_005456.1