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Antibodies > AKT1 > LS-C800192
 
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Catalog Number Size Price
LS-C800192-100 100 µl (1 mg/ml) $379 
LS-C800192-200 200 µl (1 mg/ml) $421 
AKT1 Antibody - 1:200 staining human lung carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AKT1 Antibody - 1:200 staining human lung carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AKT1 Antibody - Phospho-Akt(Ser473) antibody for IHC in human brain tissue
AKT1 Antibody
AKT1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
AKT1 Antibody - Western blot analysis on 293 cell lysates using Phospho-Akt(Ser473) antibody. The lane on the left is treated with the antigen-specific peptide.
AKT1 Antibody - Western blot analysis of Phospho-Akt(Ser473) antibody expression in Various cells lysates.
AKT1 Antibody - 1:200 staining human lung carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AKT1 Antibody - 1:200 staining human lung carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AKT1 Antibody - Phospho-Akt(Ser473) antibody for IHC in human brain tissue
AKT1 Antibody
AKT1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
AKT1 Antibody - Western blot analysis on 293 cell lysates using Phospho-Akt(Ser473) antibody. The lane on the left is treated with the antigen-specific peptide.
AKT1 Antibody - Western blot analysis of Phospho-Akt(Ser473) antibody expression in Various cells lysates.
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Polyclonal Rabbit anti‑Human AKT1 Antibody (phospho‑Ser473, IHC, IF, WB) LS‑C800192

Polyclonal Rabbit anti‑Human AKT1 Antibody (phospho‑Ser473, IHC, IF, WB) LS‑C800192

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Antibody:
AKT1 Rabbit anti-Human Polyclonal (pSer473) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800192-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AKT1 Rabbit anti-Human Polyclonal (pSer473) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
AKT1 antibody LS-C800192 is an unconjugated rabbit polyclonal antibody to AKT1 (pSer473) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Akt around the phosphorylation site of Sersine 473.
Epitope
pSer473
Specificity
Phospho-Akt(Ser473) Antibody detects endogenous levels of Akt only when phosphorylated at Sersine 473.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT1
P31749 NM_005163 NP_005154.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
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United States

Tel: (800) 227-6666 / (650) 697-3600

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For technical assistance:
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