Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
Specificity
Specific for human and mouse AKT phosphorylated at Thr308. Expected to cross-react with phosphorylated AKT (Thr308) from most vertebrate sources based on 100% sequence homology with the immunizing peptide.
Applications
- IHC
- IHC - Paraffin
- Western blot (1:500 - 1:3000)
- Immunoprecipitation
- ELISA (1:20000)
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Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. ELISA: 1:20000. Shows minimal reactivity against non-phosphorylated form of the immunizing peptide. Western Blot: 1:500-1:3000. Immunohistochemistry: 20 ug/ml. Use formalin-fixed, paraffin-embedded tissue sections; no pre-treatment of sample required.
Presentation
PBS, pH 7.2, 0.01% Sodium Azide
Storage
May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee