Human AKT1

Mouse

Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)

Chimpanzee, Mouse, Rat (at least 90% immunogen sequence identity)

IgG1,k Monoclonal

17F6.B11

Biotin

Protein A affinity chromatography

Unmodified

Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.

pSer473

This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.

• IHC (20 µg/ml)
• Immunofluorescence (1:500 - 1:3000)
• Western blot (1:500 - 1:3000)
• Immunoprecipitation
• Flow Cytometry
• ELISA (1:20000)

Biotin Conjugated Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kD in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P31749 NCBI: NM_005163 NP_005154.2