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Antibodies > AKT1 > LS-C801072
 
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Catalog Number Size Price
LS-C801072-100 100 µl (1 mg/ml) $379 
LS-C801072-200 200 µl (1 mg/ml) $421 
AKT1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
AKT1 Antibody - Western blot analysis of Phospho-Akt (Ser129) antibody expression in PMA treated A549 cells lysates. The lane on the right is treated with the antigen-specific peptide.
AKT1 Antibody - Western blot analysis of Akt phosphorylation expression in PMA treated A549 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
AKT1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
AKT1 Antibody - Western blot analysis of Phospho-Akt (Ser129) antibody expression in PMA treated A549 cells lysates. The lane on the right is treated with the antigen-specific peptide.
AKT1 Antibody - Western blot analysis of Akt phosphorylation expression in PMA treated A549 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human AKT1 Antibody (phospho‑Ser129, IF, WB) LS‑C801072

Polyclonal Rabbit anti‑Human AKT1 Antibody (phospho‑Ser129, IF, WB) LS‑C801072

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Antibody:
AKT1 Rabbit anti-Human Polyclonal (pSer129) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801072-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AKT1 Rabbit anti-Human Polyclonal (pSer129) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
AKT1 antibody LS-C801072 is an unconjugated rabbit polyclonal antibody to AKT1 (pSer129) from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Akt around the phosphorylation site of Serine 129.
Epitope
pSer129
Specificity
Phospho-Akt (Ser129) Antibody detects endogenous levels of Akt only when phosphorylated at Serine 129.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT1
P31749 NM_005163 NP_005154.2

Publications (0)

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AKT1 Antibody - Anti-AKT Monoclonal Antibody - Western Blot. anti-AKT Monoclonal Antibody in western blot shows detection of AKT (indicated by arrowhead at ~56 kD) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO (B501-0500 in TBS for 2h at RT. The membrane was probed with Anti-AKT antibody at a 1:1000 dilution in TBS with 5% BLOTTO, overnight at 4° C. For detection HRP conjugated Gt-a-Mouse IgG (p/n LS-C60680) was used at a 1:40000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
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Species: Human, Chimpanzee, Mouse, Rat
Applications: IHC, Western blot, Flow Cytometry, ELISA
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Species: Human, Mouse
Applications: IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
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Applications: Western blot, ELISA
See all AKT1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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Tel: (800) 227-6666 / (650) 697-3600

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