Human AKT1

Mouse

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

14E5.A2.B2.H9

Allophycocyanin

Protein A affinity chromatography

Unmodified

Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development.

Internal

The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.

• IHC
• Immunofluorescence
• Western blot
• Flow Cytometry (1 µg/ml)
• ELISA
• Dot Blot

Applications should be user optimized.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl

Reconstitute in 50µL of deionized water.

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P31749 NCBI: NM_005163 NP_005154.2