Human AKT1

Mouse

Human, Chimpanzee, Mouse, Rat (tested or 100% immunogen sequence identity)

Chimpanzee, Rat (at least 90% immunogen sequence identity)

IgG Monoclonal

14E5.16C8.25F6

Unconjugated

Protein A affinity chromatography

Unmodified

Anti-AKT Antibody was produced by repeated immunizations in mice with a synthetic peptide corresponding to residues internal to the human AKT1 protein.

Internal

This antibody is specific for human and mouse AKT protein. A BLAST analysis was used to suggest cross-reactivity with AKT1, AKT2, and AKT3 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT1, 2, 3 was determined with Western Blot. Cross reactivity of AKT from other sources has not been determined.

• IHC (20 µg/ml)
• Western blot (1:500 - 1:3000)
• Flow Cytometry
• ELISA (1:20000)

Applications should be user optimized.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P31749 NCBI: NM_005163 NP_005154.2