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Antibodies > AKT1 > LS-C351854
 
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Catalog Number Size Price
LS-C351854-100 100 µl (1 mg/ml) $299 
AKT1 Antibody - Immunohistochemical analysis of AKT staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
AKT1 Antibody - Western blot analysis of AKT expression in A549 (A); mouse brain (B); rat lung (C) whole cell lysates.
AKT1 Antibody - Immunohistochemical analysis of AKT staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
AKT1 Antibody - Western blot analysis of AKT expression in A549 (A); mouse brain (B); rat lung (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human AKT1 Antibody (C‑Terminus, IHC, WB) LS‑C351854

Polyclonal Rabbit anti‑Human AKT1 Antibody (C‑Terminus, IHC, WB) LS‑C351854

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Antibody:
AKT1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C351854-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AKT1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
AKT1 antibody LS-C351854 is an unconjugated rabbit polyclonal antibody to AKT1 (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, IP and WB.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human AKT.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of AKT protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT1
P31749 NM_005163 NP_005154.2

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AKT1 Antibody (aa121‑170) LS‑B1510
Species: Human, Baboon, Frog
Applications: IHC, IHC - Paraffin, Western blot
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AKT1 Antibody (phospho‑Thr450, PE) LS‑C252845
Species: Human
Applications: ELISA, Dot Blot
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AKT1 Antibody (phospho‑Thr308) LS‑C122634
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
AKT1 Antibody - Anti-AKT Monoclonal Antibody - Western Blot. anti-AKT Monoclonal Antibody in western blot shows detection of AKT (indicated by arrowhead at ~56 kD) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO (B501-0500 in TBS for 2h at RT. The membrane was probed with Anti-AKT antibody at a 1:1000 dilution in TBS with 5% BLOTTO, overnight at 4° C. For detection HRP conjugated Gt-a-Mouse IgG (p/n LS-C60680) was used at a 1:40000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
AKT1 Antibody (Internal, clone 14E5.16C8.25F6) LS‑C154001
Species: Human, Chimpanzee, Mouse, Rat
Applications: IHC, Western blot, Flow Cytometry, ELISA
Product
AKT1 Antibody (aa428‑457, Azide‑free, HRP) LS‑C251144
Species: Human, Mouse
Applications: IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Product
AKT1 Antibody (aa438‑468, Azide‑free, HRP) LS‑C275023
Species: Human
Applications: Western blot, ELISA
See all AKT1 Antibodies

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Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
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