Human AKT1 + AKT2 + AKT3

Rabbit

Human, Mouse, Rat, Chicken (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Delipidated and defibrinated

Unmodified

AKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. A BLAST analysis was used to suggest reactivity with this protein from rat, mouse, and chicken based on 100% homology for the immunogen sequence.

aa460-480

Reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.

• IHC (1:500 - 1:2000)
• Immunofluorescence (1:100 - 1:1000)
• Western blot (1:500 - 1:2000)
• Flow Cytometry
• ELISA (1:2000 - 1:10000)

Applications should be user optimized.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.