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Antibodies > AK2 / Adenylate Kinase 2 > LS-C392743
 
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Catalog Number Size Price
LS-C392743-200 200 µl $393 
AK2 / Adenylate Kinase 2 Antibody - Antibody staining Zebrafish ak2 in zebra fish body tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
AK2 / Adenylate Kinase 2 Antibody - Antibody staining Zebrafish ak2 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
AK2 / Adenylate Kinase 2 Antibody - All lanes: Anti-Zebrafish ak2 Antibody (Center) at 1:2000 dilution. Lane 1: Zebrafish lysates. Lane 2: ZF4 whole cell lysates. Lane 3: human heart lysates. Lane 4: mouse liver lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
AK2 / Adenylate Kinase 2 Antibody - Overlay histogram showing ZF4 cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
AK2 / Adenylate Kinase 2 Antibody - Antibody staining Zebrafish ak2 in zebra fish body tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
AK2 / Adenylate Kinase 2 Antibody - Antibody staining Zebrafish ak2 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
AK2 / Adenylate Kinase 2 Antibody - All lanes: Anti-Zebrafish ak2 Antibody (Center) at 1:2000 dilution. Lane 1: Zebrafish lysates. Lane 2: ZF4 whole cell lysates. Lane 3: human heart lysates. Lane 4: mouse liver lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
AK2 / Adenylate Kinase 2 Antibody - Overlay histogram showing ZF4 cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Polyclonal Rabbit anti‑Zebrafish AK2 / Adenylate Kinase 2 Antibody (aa147‑183, IHC, WB) LS‑C392743

Polyclonal Rabbit anti‑Zebrafish AK2 / Adenylate Kinase 2 Antibody (aa147‑183, IHC, WB) LS‑C392743

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Antibody:
AK2 / Adenylate Kinase 2 Rabbit anti-Zebrafish Polyclonal (aa147-183) Antibody
Application:
IHC, IHC-P, WB, Flo, Peptide-ELISA
Reactivity:
Zebrafish, Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C392743-200
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AK2 / Adenylate Kinase 2 Rabbit anti-Zebrafish Polyclonal (aa147-183) Antibody
Application:
IHC, IHC-P, WB, Flo, Peptide-ELISA
Reactivity:
Zebrafish, Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Adenylate Kinase 2 antibody LS-C392743 is an unconjugated rabbit polyclonal antibody to Adenylate Kinase 2 (AK2) (aa147-183) from zebrafish. It is reactive with human, mouse and zebrafish. Validated for Flow, IHC, Peptide-ELISA and WB.
Target
Zebrafish AK2 / Adenylate Kinase 2
Synonyms
AK2 | Adenylate kinase 2 | ADK2 | AK 2 | ATP-AMP transphosphorylase 2
Host
Rabbit
Reactivity
Zebrafish, Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa147-183
Specificity
This Zebrafish ak2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 147-183 amino acids from the Central region of Zebrafish ak2.
Applications
  • IHC
  • IHC - Paraffin (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • Peptide Enzyme-Linked Immunosorbent Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AK2 / Adenylate Kinase 2
P54819 NM_001625 NP_001616.1

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Featured Products

AK2 / Adenylate Kinase 2 Antibody - AK2 antibody Western blot of RPMI-8226 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
AK2 / Adenylate Kinase 2 Antibody (aa143‑192) LS‑C116319
Species: Human, Mouse
Applications: Western blot
AK2 / Adenylate Kinase 2 Protein
Human AK2 / Adenylate Kinase 2 Protein (Recombinant His) - LS-G481
Source: E. coli
Tag: His
See all AK2 / Adenylate Kinase 2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/26/2024
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