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Catalog Number	Size	Price
LS-C54458-100	100 µg (0.5 mg/ml)	$503

AGRP Antibody - Goat Anti-AGRP / Agouti-related protein Antibody (0.05µg/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.

Polyclonal Goat anti‑Human AGRP Antibody (aa119‑132, IHC) LS‑C54458

Name: Anti-AGRP Antibody | Goat anti-Human Polyclonal IHC,Peptide-ELISA | LSBio
Brand: Lifespan BioSciences
SKU: LS-C54458-100
Price: 503.0000 USD
Availability: InStock

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Antibody:

AGRP Goat anti-Human Polyclonal (aa119-132) Antibody

Application:

IHC-Fr, Peptide-ELISA

Reactivity:

Human, Monkey, Bat, Horse

Format:

Unconjugated, Unmodified

$503

LS-C54458-100

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

AGRP Goat anti-Human Polyclonal (aa119-132) Antibody

Application:

IHC-Fr, Peptide-ELISA

Reactivity:

Human, Monkey, Bat, Horse

Format:

Unconjugated, Unmodified

Specifications

Description

AGRP antibody LS-C54458 is an unconjugated goat polyclonal antibody to AGRP (aa119-132) from human. It is reactive with human, bat, horse and other species. Validated for IHC and Peptide-ELISA.

Target

Human AGRP

Synonyms

See All AGRP Antibodies

Host

Goat

Reactivity

Human, Monkey, Bat, Horse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence CRKLGTAMNPCSRT, from the C-Terminus of protein sequence according to NP_001129.1NP_015531.1.

Epitope

aa119-132

Specificity

Human AGRP. This antibody is expected to recognise both reported isoforms (as represented by NP_001129; NP_015531)

Applications

IHC - Frozen (0.05 - 0.1 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:32000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Preliminary experiments gave bands at approx 45kDa and 24kDa in Human Brain lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.4kDa according to NP_001129.1and NP_015531.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About AGRP

Uniprot: O00253 NCBI: NM_001138 NP_001129.1