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Catalog Number Size Price
LS-C815838-100 100 µl $412 
AFG3L2 Antibody - Immunochemical staining of human AFG3L2 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
AFG3L2 Antibody - Immunochemical staining of human AFG3L2 in human duodenum with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
AFG3L2 Antibody - Immunofluorescence staining of AFG3L2 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AFG3L2 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
AFG3L2 Antibody - Anti-AFG3L2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MDA-MB453 Whole Cell Lysate. Lane C: MCF-7 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 89 kDa. Observed band size: 95 kDa. (We are unsure as to the identity of these extra bands.)
AFG3L2 Antibody - AFG3L2 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate. 4 uL anti-AFG3L2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-AFG3L2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 89 kDa. Observed band size: 95 kDa.
AFG3L2 Antibody - Immunochemical staining of human AFG3L2 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
AFG3L2 Antibody - Immunochemical staining of human AFG3L2 in human duodenum with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
AFG3L2 Antibody - Immunofluorescence staining of AFG3L2 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AFG3L2 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
AFG3L2 Antibody - Anti-AFG3L2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MDA-MB453 Whole Cell Lysate. Lane C: MCF-7 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 89 kDa. Observed band size: 95 kDa. (We are unsure as to the identity of these extra bands.)
AFG3L2 Antibody - AFG3L2 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate. 4 uL anti-AFG3L2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-AFG3L2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 89 kDa. Observed band size: 95 kDa.
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Polyclonal Rabbit anti‑Human AFG3L2 Antibody (IHC, IF, WB) LS‑C815838

Polyclonal Rabbit anti‑Human AFG3L2 Antibody (IHC, IF, WB) LS‑C815838

Antibody:
AFG3L2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$412
LS-C815838-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
AFG3L2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
AFG3L2 antibody LS-C815838 is an unconjugated rabbit polyclonal antibody to human AFG3L2. Validated for ICC, IF, IHC, IP and WB.
Target
Human AFG3L2
Synonyms
AFG3L2 | AFG3-like protein 2 | SPAX5 | Spinocerebellar ataxia 28 | Paraplegin-like protein | ATPase family gene 3, yeast | SCA28
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
E. coli-derived Human AFG3L2 fragment
Specificity
Human AFG3L2
Applications
  • IHC - Paraffin (1:500 - 1:2000)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.0, 0.03% Proclin 300
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AFG3L2
Q9Y4W6 NM_006796 NP_006787.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024