Human ADRBK1/2

Mouse

Human, Mouse, Rat, Bovine, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein G purified

Unmodified

Partial GST fusion protein corresponding to the GRK3 (bARK2) C-Terminus.

C-Terminus

Recognizes GST fusion to GRK3 C-terminus (aa467-688), homologous to GRK2; epitope maps to aa483-485. Species cross-reactivity: Rat, mouse, chicken, bovine, porcine and rabbit.

• Western blot (0.1 - 1 µg/ml)
• Immunoprecipitation

WB: Detects GRK2/3 in RIPA lysates from mouse 3T3 fibroblasts. Previous lots of this antibody detected GRK2/3 in bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma and human HeLa cell RIPA lysates. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with Mouse Anti-GRK 2,3 (0.1 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. IP: Can immunoprecipitate GRK2/3 from transfected cells.

Tris-Glycine, pH 7.4, 0.05% Sodium Azide, 40% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.