Target
Dog ADORA2A/Adenosine A2A Receptor
Synonyms
ADORA2A | Adenosine receptor A2a | ADORA2 | A2a receptor | Adenosine A2a receptor | Adenosine A2 receptor | Adenosine receptor subtype A2a | HA2aR | RDC8 | AA2AR | A2AR
Reactivity
Dog, Human, Rat, Bovine, Pig
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Amino acid sequence near the 4th C-Terminus intracellular domain (30 amino acids) of the cloned canine A2a receptor. The A2a sequence selected is not present in A1, A2b and A3 receptors.
Specificity
Recognizes canine Adenosine A2a receptor. Recognizes a band of ~45kD in human (coronary artery), and rat, porcine and bovine striatal membranes. Species cross-reactivity: human, rat, bovine and porcine.
Applications
- IHC
- IHC - Frozen (10 - 50 µg/ml)
- Immunofluorescence
- Western blot (1 - 10 µg/ml)
- ELISA (1:10000 - 1:50000)
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Usage
Suitable for use in ELISA, Immunohistochemistry and Western Blot. ELISA: 1:10000-1:50000. Immunohistochemistry (Frozen): 10-50 ug/ml. Western Blot: 1-10 ug/ml (Chemiluminescence technique), 40-100 ug membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
Presentation
PBS, pH 7.4, 0.05% Sodium Azide, 0.1% BSA
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADORA2A/Adenosine A2A Receptor