Target
Rat ADORA1 / Adenosine A1 Receptor
Synonyms
ADORA1 | A1 adenosine receptor | A1ar | Adenosine A1 receptor | Adenosine receptor A1 | ADO-A1 receptor | RDC7
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic peptide 14aa sequence corresponding to rat A1R gene. This region corresponds to the 3rd extracellular domain, N-Terminus. A cysteine has been added at the NH2 terminus for coupling to KLH.
Specificity
Recognizes Adenosine A1 Receptor. Species cross-reactivity: Rat, porcine, lamb. Species sequence Homology: Mouse, canine, rabbit and human: 92%; bovine: 78%.
Applications
- Western blot (1 - 10 µg/ml)
- ELISA (1:10000 - 1:50000)
Usage
Suitable for use in ELISA and Western Blot. Western Blot: 1-10 ug/ml using Detects 79 and 39kD bands in cortical membranes from rat, pig, and lamb. The 79kD band can be converted to 39kD band by agonist or antagonist treatment and could represent a A1R dimers. A1R, photoaffinity labeled with 125I-R-AHPIA as 39kD band. ELISA: 1:10000-1:50000 using 50-100 ng A1R control peptide/well.
Presentation
100 mM Tris, pH 7.5, 0.05% Sodium Azide, 40% Glycerol, 0.2% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADORA1 / Adenosine A1 Receptor