Reactivity
Adenovirus
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
TrpE-Ad2 E1A fusion protein isolated from E. coli.
Specificity
Recognizes the three adenovirus (Ad) 2 and 5 E1A gene products, Mr 36-50kD.
Applications
- ICC (0.1 µg/ml)
- Western blot
- Immunoprecipitation
- ELISA
Usage
Suitable for use in ELISA, Immunoprecipitation, Immunocytochemistry and Western Blot. Western Blot Analysis: 0.05-0.1 ug/ml detects the Ad2 and Ad5 E1A gene products in RIPA lysates from 293 cells. 293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti- E1A (Ad2/Ad5) (0.1 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: An independent laboratory has reported that this antibody immunoprecipitates Ad2 and Ad5 E1A gene products and associated proteins. Immunocytochemistry: An independent laboratory has reported that this antibody immunostains Ad2 and Ad5 E1A gene products.
Presentation
0.05 M Sodium Phosphate, pH 7.4, 0.1% Sodium Azide, 0.2% Gelatin
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee