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Vector Laboratories, Inc.
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Catalog Number Size Price
LS-C806866-100 100 µl (1 mg/ml) $379 
LS-C806866-200 200 µl (1 mg/ml) $421 
ADAP / FYB Antibody - 1:100 staining mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ADAP / FYB Antibody - Staining COLO cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
ADAP / FYB Antibody - Western blot analysis of FYB using NIH-3T3 whole lysates.
ADAP / FYB Antibody - 1:100 staining mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ADAP / FYB Antibody - Staining COLO cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
ADAP / FYB Antibody - Western blot analysis of FYB using NIH-3T3 whole lysates.
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Polyclonal Rabbit anti‑Human ADAP / FYB Antibody (IHC, IF, WB) LS‑C806866

Polyclonal Rabbit anti‑Human ADAP / FYB Antibody (IHC, IF, WB) LS‑C806866

Antibody:
ADAP / FYB Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C806866-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ADAP / FYB Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
FYB antibody LS-C806866 is an unconjugated rabbit polyclonal antibody to FYB (ADAP) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human ADAP / FYB
Synonyms
FYB | ADAP | FYN-binding protein | FYN-T-binding protein | FYB-120/130 | FYN binding protein | p120/p130 | PRO0823 | SLAP130 | SLAP-130
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
Synthesized peptide derived from human Fyb
Specificity
FYB Antibody detects endogenous levels of total FYB.
Applications
  • IHC
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:1000 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADAP / FYB
O15117 NM_001465 NP_001456.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024