Human ADAM10

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

163003

Allophycocyanin. Also available conjugated with CF.

Purified

Unmodified

Mouse myeloma cell line NS0-¬derived recombinant human ADAM10 (aa Thr214-Glu672; UniProt Accession #O14672).

Recognizes human ADAM10 (APC).

• Flow Cytometry
• Applications tested for the base form of this product only

Suitable for use in Flow Cytometry Flow Cytometry: Intracellular staining antibodies are designed for multiparameter flow cytometric analysis of cells. To stain for surface proteins (e. g. CD3, CD4, CD8) in addition to the intracellular protein, we recommend that the investigator determine whether the fixation and permeabilization steps adversely affect the surface protein. If so, surface staining of cells prior to fixation and permeabilization is recommended. For intracellular staining, cells must first be fixed and permeabilized. Use of 4% paraformaldehyde in PBS as a fixative is recommended. Other formulations or tissue fixatives may affect the staining properties of the monoclonal antibody. For permeabilization, 0.1% saponin in a balanced salt solution is effective in facilitating antibody entry into cells. Due to the reversible nature of cell membrane permeabilization, saponin must be included in all buffers used (i. e. both the staining and washing steps). 10ul of undiluted product can be used to stain 1 x 10e6 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from PBS, 0.1% Sodium Azide

40-50% glycerol, PBS

May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for up to 3 months at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O14672 NCBI: NM_001110 NP_001101.1