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Catalog Number Size Price
LS-C662668-10 10 µg $318 
LS-C662668-100 100 µg $470 
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Western blot analysis of ADA using anti-ADA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human 22RV1 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADA at approximately 45KD. The expected band size for ADA is at 40KD.
ADA / Adenosine Deaminase Antibody - Western blot - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Western blot analysis of ADA using anti-ADA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human 22RV1 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADA at approximately 45KD. The expected band size for ADA is at 40KD.
ADA / Adenosine Deaminase Antibody - Western blot - Anti-ADA/Adenosine Deaminase Picoband antibody
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Polyclonal Rabbit anti‑Mouse ADA / Adenosine Deaminase Antibody (IHC, WB) LS‑C662668

Polyclonal Rabbit anti‑Mouse ADA / Adenosine Deaminase Antibody (IHC, WB) LS‑C662668

Antibody:
ADA / Adenosine Deaminase Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662668-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ADA / Adenosine Deaminase Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Adenosine Deaminase antibody LS-C662668 is an unconjugated rabbit polyclonal antibody to mouse Adenosine Deaminase (ADA). Validated for ELISA, IHC and WB.
Target
Mouse ADA / Adenosine Deaminase
Synonyms
ADA | Adenosine deaminase | ADA1 | Adenosine aminohydrolase
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived mouse ADA recombinant protein (Position: A2-H238).
Specificity
Detected in brain neurons in the median emninence (at protein level) (PubMed:8783262). Expressed in secondary deciduum (at protein level) (PubMed:9272950). Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADA / Adenosine Deaminase
P00813 NM_000022 NP_000013.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/16/2024