Chicken Actin

Mouse

Chicken, Human, Mouse, Rat, Guinea pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)

IgG2b,k Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Purified chicken gizzard actin. Epitope: Appears to be located in the N-Terminus two thirds of the actin molecule, possibly near aa50-70.

N-Terminus

Recognizes all six isoforms of vertebrate actin, globular (G) and filamentous (F) forms. This clone is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. Labels myotubes and stains myoblasts and fibroblasts. Does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. Species cross-reactivity: All species.

• IHC
• IHC - Paraffin
• ICC (1 - 20 µg/ml)
• Western blot
• ELISA

Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA. Immunohistochemistry: 10 ug/ml (paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections). Visualized using a donkey anti-mouse IgG (Cy3). Positive staining pattern reflects both membrane and cytoplasmic staining. ELISA: Shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows significant binding to both Dictyostelium discoideum and Physarum polycephalum in ELISA.

0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% Sodium Azide

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.