Chicken Actin

Mouse

Chicken (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated

Ascites

Unmodified

Purified chicken gizzard actin.

aa50-70

Appears to be specific for the N-terminal two-thirds of the actin molecule (possibly near residues 50-70 in primary sequence). Reacts with every actin form tested. Species cross-reactivity: Reacts with all animal species and cell types with an actin form, including plant actin.

• IHC
• IHC - Frozen
• Immunofluorescence (1:100)
• Western blot (1:100 - 1:1000)
• ELISA

Suitable for use in Indirect Immunofluorescence, Western Blot, Iodination and Solid Phase Binding. Indirect immunofluorescence: 1:100; Tissue culture cells-fix with formaldehyde, treat with methanol or acetone. Glycerinated myofibrils-fix fibers with formaldehyde, treat with cold methanol. Stains I-bands intensely and stress fibers in human fibroblasts. Cryostat sections (6um)-quick frozen in isopentane, slides treated with gelatin and formaldehyde. Western Blots: 1:100-1:1000; On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle. Solid Phase Binding Assay: 1:800-1:1000; In an ELISA, is strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoideum and Physarum polycephalum.

0.01% Sodium Azide

For research use only. Intended for use by laboratory professionals.

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