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Antibodies > ABL1 / c-ABL > LS-C800899
 
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Catalog Number Size Price
LS-C800899-100 100 µl (1 mg/ml) $379 
LS-C800899-200 200 µl (1 mg/ml) $421 
ABL1 / c-ABL Antibody - 1:200 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ABL1 / c-ABL Antibody - Staining COS7 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ABL1 / c-ABL Antibody - Staining K562 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
ABL1 / c-ABL Antibody - Western blot analysis of Abl phosphorylation expression in RAW264.7 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
ABL1 / c-ABL Antibody - 1:200 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ABL1 / c-ABL Antibody - Staining COS7 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ABL1 / c-ABL Antibody - Staining K562 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
ABL1 / c-ABL Antibody - Western blot analysis of Abl phosphorylation expression in RAW264.7 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human ABL1 / c‑ABL Antibody (phospho‑Tyr393,phospho‑Tyr412, IHC, IF, WB) LS‑C800899

Polyclonal Rabbit anti‑Human ABL1 / c‑ABL Antibody (phospho‑Tyr393,phospho‑Tyr412, IHC, IF, WB) LS‑C800899

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Antibody:
ABL1 / c-ABL Rabbit anti-Human Polyclonal (pTyr393,pTyr412) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800899-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ABL1 / c-ABL Rabbit anti-Human Polyclonal (pTyr393,pTyr412) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
C-ABL antibody LS-C800899 is an unconjugated rabbit polyclonal antibody to c-ABL (ABL1) (pTyr393,pTyr412) from human. It is reactive with human, mouse and monkey. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human ABL1 / c-ABL
Synonyms
ABL1 | Bcr/c-abl oncogene protein | Bcr-Abl | Bcr/abl | C-ABL | ABL | JTK7 | p150 | Tyrosine-protein kinase ABL1 | Proto-oncogene c-Abl | V-abl
Host
Rabbit
Reactivity
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Abl around the phosphorylation site of Tyrosine 393/412.
Epitope
pTyr393,pTyr412
Specificity
Phospho-Abl (Tyr393/412) Antibody detects endogenous levels of Abl only when phosphorylated at Tyrosine 393/412.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ABL1 / c-ABL
P00519 NM_005157 NP_005148.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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