LSBio logo with text
Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers


Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > ABL1 / c-ABL > LS-C800901
 
Print Page  
Catalog Number Size Price
LS-C800901-100 100 µl (1 mg/ml) $379 
LS-C800901-200 200 µl (1 mg/ml) $421 
ABL1 / c-ABL Antibody - 1:200 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22° C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ABL1 / c-ABL Antibody - Staining COS7 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ABL1 / c-ABL Antibody - Staining K562 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
ABL1 / c-ABL Antibody - Western blot analysis of c-Abl phosphorylation expression in Insulin treated K562 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
ABL1 / c-ABL Antibody - Western blot analysis of Phospho-c-Abl (Tyr245) antibody expression in Insulin treated K562 cells lysates. The lane on the right is treated with the antigen-specific peptide.
ABL1 / c-ABL Antibody - 1:200 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22° C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ABL1 / c-ABL Antibody - Staining COS7 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ABL1 / c-ABL Antibody - Staining K562 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
ABL1 / c-ABL Antibody - Western blot analysis of c-Abl phosphorylation expression in Insulin treated K562 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
ABL1 / c-ABL Antibody - Western blot analysis of Phospho-c-Abl (Tyr245) antibody expression in Insulin treated K562 cells lysates. The lane on the right is treated with the antigen-specific peptide.
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5

Polyclonal Rabbit anti‑Human ABL1 / c‑ABL Antibody (phospho‑Tyr245, IF, WB) LS‑C800901

Polyclonal Rabbit anti‑Human ABL1 / c‑ABL Antibody (phospho‑Tyr245, IF, WB) LS‑C800901

Submit Review LSBio Guarantee
Antibody:
ABL1 / c-ABL Rabbit anti-Human Polyclonal (pTyr245) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800901-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ABL1 / c-ABL Rabbit anti-Human Polyclonal (pTyr245) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
C-ABL antibody LS-C800901 is an unconjugated rabbit polyclonal antibody to c-ABL (ABL1) (pTyr245) from human. It is reactive with human, mouse, rat and other species. Validated for IF, Peptide-ELISA and WB.
Target
Human ABL1 / c-ABL
Synonyms
ABL1 | Bcr/c-abl oncogene protein | Bcr-Abl | Bcr/abl | C-ABL | ABL | JTK7 | p150 | Tyrosine-protein kinase ABL1 | Proto-oncogene c-Abl | V-abl
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human c-Abl around the phosphorylation site of Tyrosine 245.
Epitope
pTyr245
Specificity
Phospho-c-Abl (Tyr245) Antibody detects endogenous levels of c-Abl only when phosphorylated at Tyrosine 245.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ABL1 / c-ABL
P00519 NM_005157 NP_005148.2

Publications (0)

Submit a Publication for LS-C800901

Customer Reviews (0)

Submit a review for LS-C800901

Featured Products

ABL1 / c-ABL Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
ABL1 / c‑ABL Antibody (phospho‑Tyr412) LS‑C178071
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Product
ABL1 / c‑ABL Antibody (aa613‑641, Azide‑free, Biotin) LS‑C250586
Species: Mouse
Applications: IHC, Western blot, ELISA
ABL1 / c-ABL Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ ABL1 / c‑ABL Antibody LS‑B13844
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Product
ABL1 / c‑ABL Antibody (phospho‑Thr735) LS‑C12202
Species: Human
Applications: Western blot
Product
ABL1 / c‑ABL Antibody (phospho‑Tyr412) LS‑C122765
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Product
ABL1 / c‑ABL Antibody LS‑C122769
Species: Human, Mouse
Applications: IHC
See all ABL1 / c-ABL Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2024 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

To place an order:
customerservice@vectorlabs.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com