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Catalog Number	Size	Price
LS-C312249-10	10 µg	$318
LS-C312249-100	100 µg (0.5 mg/ml)	$470

ABI1 / SSH3BP1 Antibody - IHC analysis of ABI using anti-ABI antibody. ABI was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ABI Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

ABI1 / SSH3BP1 Antibody - IHC analysis of ABI using anti-ABI antibody. ABI was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ABI Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

ABI1 / SSH3BP1 Antibody - ABI1 / ABI-1 antibody. IHC(P): Rat Brain Tissue.

ABI1 / SSH3BP1 Antibody - Western blot analysis of ABI-1 using anti-ABI-1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human THP-1 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABI-1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABI-1 at approximately 65KD. The expected band size for ABI-1 is at 55KD.

ABI1 / SSH3BP1 Antibody - WB of ABI1 / ABI-1 antibody.

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Polyclonal Rabbit anti‑Human ABI1 / SSH3BP1 Antibody (aa483‑494, IHC, WB) LS‑C312249

Name: Anti-ABI1 / SSH3BP1 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C312249-10
Price: 318.0000 USD
Availability: InStock

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Note: This antibody replaces LS-C389235

Antibody:

ABI1 / SSH3BP1 Rabbit anti-Human Polyclonal (aa483-494) Antibody

Application:

IHC, IHC-P, ICC, WB

Reactivity:

Human, Monkey, Mouse, Rat, Bat, Guinea pig, Horse, Pig, Rabbit, Sheep

Format:

Unconjugated, Unmodified

$318

LS-C312249-10

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For Research Use Only

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Overview

Antibody:

ABI1 / SSH3BP1 Rabbit anti-Human Polyclonal (aa483-494) Antibody

Application:

IHC, IHC-P, ICC, WB

Reactivity:

Human, Monkey, Mouse, Rat, Bat, Guinea pig, Horse, Pig, Rabbit, Sheep

Format:

Unconjugated, Unmodified

Specifications

Description

SSH3BP1 antibody LS-C312249 is an unconjugated rabbit polyclonal antibody to SSH3BP1 (ABI1) (aa483-494) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IHC and WB.

Target

Human ABI1 / SSH3BP1

Synonyms

See All ABI1 / SSH3BP1 Antibodies

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat, Bat, Guinea pig, Horse, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the C-Terminus of human ABI1 (483-494 aa WYEGVCNRVTGL), different from the related rat and mouse sequences by one amino acid.

Epitope

aa483-494

Specificity

Widely expressed, with highest expression in brain. .

Applications

IHC
IHC - Paraffin
ICC
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ABI1 / SSH3BP1

Uniprot: Q8IZP0 NCBI: NM_005470 NP_005461.2