Human ABCC6 / MRP6

Rat

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

M6II-31

Unconjugated

Tissue culture supernatant

Concentrated

M6II-31 reacts with MRP6, a 190-200 kD transmembrane protein that is related to the multidrug resistance related protein MRP. Mutations in the MRP6 gene are responsible for the connective tissue disorder Pseudoxanthoma elasticum (PXE). M6II-31 was raised against a bacterial fusion protein of human MRP6, containing amino acids 764-964, spanning the putative 12th transmembrane region as well as predicted internal and external regions of the protein. M6II-7 did not cross-react with the human MDR1, MRP1, MRP2, MRP3, MRP4, MRP5 gene products.

• IHC
• IHC - Paraffin
• IHC - Frozen
• Western blot
• Flow Cytometry

M6II-31 can be used to detect human MRP6 in cells and tissues. Immunocytochemistry: use 1:20-50 dilution on acetone fixed cytospin preparations. For immunohistochemistry: M6II-31 (use 1:20-50) on acetone fixed frozen sections can be followed by incubation with rabbit anti-rat IgG (1:25, Dako) and an APAAP complex (1:50, Dako). M6II-31 can be used on formaldehyde-fixed paraffin-embedded human tissues, after pretreatment with 0.01 M citric acid (pH 6.0) in distilled water at 100 C for 10 minutes. After incubation of M6II-31 (use 1:20) and washing, slides can be incubated with biotinylated rabbit anti-rat IgG (1:100, Jackson, West Grove) and streptavidin conjugated to horseradish peroxidase (1:500, Zymed, San Francisco, CA). Flow cytometry: optimal conditions still to be defined. Western blotting: use 1:20-50 dilution and anti-rat-HRP.

Tissue Culture Supernatant, 0.1% Sodium Azide, 0.7% BSA, Serum-free

Store at 2-8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O95255 NCBI: NM_001171 NP_001162.4