Human ABCB1 / MDR1 / P Glycoprotein

Mouse

Human (tested or 100% immunogen sequence identity)

Mouse, Rat

IgG2a Monoclonal

UIC2

Unconjugated

Affinity purified

Unmodified

Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA.

Recognizes an extracellular conformational epitope of CD243, also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein), a transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds. CD243 is expressed at low levels in the cell membrane of peripheral blood leucocytes, and constitutively expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. Strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs. Can be used in a shift assay to selectively demonstrate the expression and functional activity of CD243 in a target cell. Does not cross-react with mitochondrial pyruvate carboxylase.

• IHC
• IHC - Paraffin
• IHC - Frozen
• Immunofluorescence
• Immunoprecipitation
• Flow Cytometry (1:50 - 1:200)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Immunohistochemistry: This product may benefit from antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose (3-4). Histology Positive Control Tissue: Human kidney.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08183 NCBI: NM_000927 NP_000918.2