Human ABCA8

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-KEQELGDFEEDFD, from the C-Terminus of protein sequence according to NP_009099.1.

aa1596-1608

Human ABCA8. This antibody is expected to cross-react with the highly similar Human ABCA9, since the immunizing peptide differs from ABCA9 with only one residue (F8).

• Peptide Enzyme-Linked Immunosorbent Assay (1:4000)

Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Preliminary experiments gave an approx. 50-55kD band in Human Brain (Cerebellum, Cerebral Cortex, Frontal Cortex), Heart, Skeletal Muscle and Testis lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 179kD according to NP_009099.1. The 50-55kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O94911 NCBI: NM_007168 NP_009099.1