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Antibodies > 4-HNE > LS-C775480
 
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Catalog Number Size Price
LS-C775480-100 100 µg (1 mg/ml) $535 
4-HNE Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
4-HNE Antibody - Western Blot analysis of 4-hydroxy-2-hexanal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-2-hexenal protein using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: 4-hydroxyl nonenal-BSA (0.5 µg). Lane 4: 4-hydroxy nonenal-BSA (2.0 µg). Lane 5: 4-hydroxy-2-hexenal (0.5 µg). Lane 6: 4-hydroxy-2-hexenal (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
4-HNE Antibody - Flow Cytometry analysis using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
4-HNE Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
4-HNE Antibody - Western Blot analysis of 4-hydroxy-2-hexanal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-2-hexenal protein using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: 4-hydroxyl nonenal-BSA (0.5 µg). Lane 4: 4-hydroxy nonenal-BSA (2.0 µg). Lane 5: 4-hydroxy-2-hexenal (0.5 µg). Lane 6: 4-hydroxy-2-hexenal (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
4-HNE Antibody - Flow Cytometry analysis using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
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Monoclonal Mouse anti‑All species 4‑HNE Antibody (clone 6F10, Atto 390, IF, WB) LS‑C775480

Monoclonal Mouse anti‑All species 4‑HNE Antibody (clone 6F10, Atto 390, IF, WB) LS‑C775480

Submit Review LSBio Guarantee
Antibody:
4-HNE Mouse Monoclonal (Atto 390) (6F10) Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
All species
Format:
Atto 390, Unmodified
Price
Catalog Number
$535
LS-C775480-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
4-HNE Mouse Monoclonal (Atto 390) (6F10) Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
All species
Format:
Atto 390, Unmodified

Specifications

Description
4-HNE antibody LS-C775480 is an Atto 390-conjugated mouse monoclonal antibody to all species 4-HNE. Validated for ELISA, Flow, IF and WB.
Target
All species 4-HNE
Host
Mouse
Reactivity
All species
Clonality
IgG2b Monoclonal
Clone
6F10
Conjugations
Atto 390
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).
Specificity
Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Applications
  • Immunofluorescence (1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:50)
  • ELISA (1:1000)
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

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Featured Products

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Applications: IHC, IHC - Paraffin, Western blot, ELISA
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4‑HNE Antibody LS‑C125731
Species: Mouse
Applications: Western blot
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4‑HNE Antibody LS‑C68183
Species:
Applications: Western blot, ELISA
4-HNE Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
4‑HNE Antibody (clone 6F10, FITC) LS‑C775485
Species: All species
Applications: Immunofluorescence, Western blot, Flow Cytometry, ELISA
4-HNE Antibody - Dot blot analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 5C11.1. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.
4‑HNE Antibody (clone 5C11.1, Atto 390) LS‑C775496
Species: All species
Applications: Western blot, ELISA
4-HNE ELISA Kit
Human 4-HNE (Competitive EIA) ELISA Kit - LS-F40036
Reactivity: Human
Range: 31.25-2000 pg/ml
See all 4-HNE Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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United States

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