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POU5F1 / OCT4

POU class 5 homeobox 1

POU5F1 / OCT4 is a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Gene Name: POU class 5 homeobox 1
Synonyms: POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3, OCT3/4
Target Sequences: NM_002701 NP_002692.2 Q01860

Publications (3)

1
Endothelial progenitor cells restore renal function in chronic experimental renovascular disease. Chade AR, Zhu X, Lavi R, Krier JD, Pislaru S, Simari RD, Napoli C, Lerman A, Lerman LO. Circulation. 2009 119:547-57. (ICC, WB, IHC-P, IHC-Fr; Pig) [PubMed:19153272] [PMC:PMC2758066]
2
Stabilization of HIF-1a and HIF-2a, up-regulation of MYCC and accumulation of stabilized p53 constitute hallmarks of CNS-PNET animal model. Malchenko S, Sredni ST, Bi Y, Margaryan NV, Boyineni J, Mohanam I, Tomita T, Davuluri RV, Soares MB. PloS one. 2017 12:e0173106. (IHC, WB; Mouse) [Full Text Article] [PubMed:28249000] [PMC:PMC5332108] Related Antibodies: LS-B85.
3
Step-by-step protocols for non-viral derivation of transgene-free induced pluripotent stem cells from somatic fibroblasts of multiple mammalian species. Sho Yoshimatsu, Atsushi Yamazaki, Kazuya Edamura, Yuko Koushige, Hisashi Shibuya, Emi Qian, Tsukika Sato, Junko Okahara, Noriyuki Kishi, Toshiaki Noce, Yoshifumi Yamaguchi, Hideyuki Okano. Development, growth & differentiation. 2022 August;64:325-341. [Full Text Article] [PubMed:35841539] Related Antibodies: LS-B1194.

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Proteins (11)
Over-Expression Lysate (4)
Recombinant (7)
POU5F1 / OCT4 (11)
No (11)
6His, N-terminus (1)
6His-B2M, N-terminus (1)
GST (1)
His (1)
His-T7 (2)
Myc-DDK (Flag) (5)
Human (8)
Mouse (1)
293T Cells (2)
E. coli (5)
HEK 293 Cells (3)
Wheat Germ Extract (1)
Purified (3)
POU5F1 / OCT4 Protein - Purified recombinant protein POU5F1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
38.4 kDa
20 µg/$1,107
POU5F1 / OCT4 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
6His-B2M, N-terminus
1 mg/$1,355; 100 µg/$420; 20 µg/$323
POU5F1 / OCT4 Protein
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E. coli
His
100 µg/$411; 500 µg/$985; 20 µg/$276
POU5F1 / OCT4 Protein - 12.5% SDS-PAGE of human POU5F1 stained with Coomassie Blue
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Wheat Germ Extract
GST
44.7 kDa
10 µg/$479; 25 µg/$670
POU5F1 / OCT4 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
38.4 kDa
100 µg/$494
POU5F1 / OCT4 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
21.1 kDa
100 µg/$494
POU5F1 / OCT4 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
38.4 kDa
20 µg/$150
POU5F1 / OCT4 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
21.1 kDa
20 µg/$150
POU5F1 / OCT4 Protein - Recombinant  Octamer Binding Transcription Factor 4 By SDS-PAGE
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E. coli
His-T7
28.8 kDa
10 µg/$281; 50 µg/$442; 100 µg/$705; 200 µg/$882; 1 mg/$1,989; 500 µg/$1,529; 5 mg/$3,081; 2 mg/$2,176
POU5F1 / OCT4 Protein - Recombinant  Octamer Binding Transcription Factor 4 By SDS-PAGE
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E. coli
His-T7
29.2 kDa
10 µg/$280; 50 µg/$437; 100 µg/$700; 200 µg/$872; 1 mg/$1,978; 500 µg/$1,519; 5 mg/$3,053; 2 mg/$2,164
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E. coli
6His, N-terminus
27.4 kDa
10 µg/$265; 50 µg/$315; 100 µg/$365
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).