Target
SHH / Sonic Hedgehog
Synonyms
SHH | HHG-1 | HLP3 | Sonic hedgehog | Sonic hedgehog homolog | MCOPCB5 | Sonic hedgehog protein | HHG1 | HPE3 | SMMCI | TPT | TPTPS
Conjugations
Unconjugated
Predicted Molecular Weight
19.8 kDa
AA Sequence
IVIGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKISRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG
Purification
Greater than 98% by SDS-PAGE and HPLC
Usage Summary
A single non-glycosylated polypeptide chain containing 176 amino acids.
Bio-Activity
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 µg/ml, corresponding to a specific activity of > 1.0 × 103 IU/mg.
Endotoxin
Less than 1 EU/µg (determined by LAL method).
Presentation
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl
Reconstitution
Briefly centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage
Store lyophilized at 2°C to 8°C for short term storage. Store lyophilized at -20°C for long term storage. Store reconstituted at 2°C to 8°C for up to 1 week. Aliquot and store reconstituted at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About SHH / Sonic Hedgehog