Target
PDCD1 / CD279 / PD-1
Synonyms
PDCD1 | CD279 | HPD-1 | HPD-l | PD1 | Protein PD-1 | SLEB2 | PD-1 | CD279 antigen | Programmed cell death 1
Modifications
Preservative Free.
Also available
Unmodified.
Conjugations
Unconjugated
Region
Extracellular domain of human CD279(PD-1) and murine IgG2a Fc +hinge.
Predicted Molecular Weight
52 kDa
Expression System
CHO Cells
Source Species
Hamster-Chinese
Usage Summary
A soluble fusion protein consisting of the extracellular (139aa) domain of human CD279(PD-1) and murine IgG2a Fc +hinge. In SDS-PAGE, this protein runs at 52 kd under native, and 27 kd under reducing conditions. Data suggest that the murine Ig Fc portion of this construct may be prone to cleavage. Five x 10^5cultured human monocyte-derived Dendritic cells (moDC) were washed and incubated 45 minutes on ice with 80 ul of CD279-muIg/Biotin at 10 µg/ml. Cells were washed twice and incubated with secondary reagent Streptavidin/PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.57 log10 fluorescent units. This binding was blocked when cells were pre incubated with 100 ug/ml of purified CD279-muIg.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl
Storage
Store at 2°C to 5°C for up to 6 months. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDCD1 / CD279 / PD-1