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| Catalog Number | Size | Price |
|---|---|---|
| LS-G145899-100 | 100 µg | $1,070 |
Coronavirus SARS-CoV-2 Spike Glycoprotein (Recombinant mFc, C-terminus) (Arg319-Phe541) - LS-G145899
Coronavirus SARS-CoV-2 Spike Glycoprotein (Recombinant mFc, C-terminus) (Arg319-Phe541) - LS-G145899
Description:
SARS-CoV-2 Spike Glycoprotein LS-G145899 is a Recombinant Coronavirus SARS-CoV-2 Spike Glycoprotein produced in HEK 293 Cells Arg319-Phe541 with mFc, C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method).
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Overview
Description:
SARS-CoV-2 Spike Glycoprotein LS-G145899 is a Recombinant Coronavirus SARS-CoV-2 Spike Glycoprotein produced in HEK 293 Cells Arg319-Phe541 with mFc, C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method).
Specifications
Type
Recombinant Protein
Target
SARS-CoV-2 Spike Glycoprotein
Synonyms
S / Spike Glycoprote | SARS-CoV-2 | SARS-Cov2 | COVID-19 | SARS-CoV2 (COVID-19) Spike
Species
Coronavirus SARS-CoV-2
Modifications
Unmodified
Conjugations
Unconjugated
Tag
mFc, C-terminus
Region
Arg319-Phe541
Predicted Molecular Weight
51.5 kDa
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 95 % as determined by reducing SDS-PAGE.
Usage Summary
A DNA sequence encoding the 2019-nCoV Spike Protein (RBD) (YP_009724390.1) (Arg319-Phe541) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2µg/mL (100µL/well) can bind Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag), the EC50 of Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag) is 15-50 ng/mL. Loaded Human ACE2 Protein (His tag) on SA Biosensor, can bind Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag) with an affinity constant of 0.562 nM as determined by Octet RED System.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4
Storage
Long term: store at -20°C to -80°C for up to Lyophilized store for up to 12 months. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted store at less than -20°C for 3 months
Restrictions
For research use only. Intended for use by laboratory professionals.
About SARS-CoV-2 Spike Glycoprotein
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Images
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
