Target
SARS-CoV-2 Spike Glycoprotein
Synonyms
S / Spike Glycoprote | SARS-CoV-2 | SARS-Cov2 | COVID-19 | SARS-CoV2 (COVID-19) Spike
Species
Coronavirus SARS-CoV-2
Conjugations
Unconjugated
Predicted Molecular Weight
26.54 kDa
Expression System
Baculovirus
Purification
Greater than 95 % as determined by reducing SDS-PAGE.
Usage Summary
A DNA sequence encoding the 2019-nCoV Spike Protein (RBD) (YP_009724390.1) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus.
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 µg/mL (100 µL/well) can bind Recombinant 2019-nCoV Spike Protein (RBD, His Tag), the EC50 of Recombinant 2019-nCoV Spike Protein (RBD, His Tag) is 35-70 ng/mL.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile 20 mM PB, pH 7.0, 300 mM NaCl, 10% Glycerol
Storage
Long term: store at -20°C to -80°C for up to Lyophilized store for up to 12 months. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted store at less than -20°C for 3 months
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About SARS-CoV-2 Spike Glycoprotein