Target
Rat SLC2A7 / GLUT7
Synonyms
SLC2A7 | Glucose transporter type 7 | GLUT-7 | GLUT7
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
A 14 AA peptide sequence near the C-Terminus of rat Glucose Transporter 7 (1). A cysteine has been added at the NH2 terminus for coupling to KLH.
Specificity
Recognizes rat GLUT 7. Species sequence homology: human. Glut-7 64%: Glut-2 68% and show similar secondary and tertiary structures. However, Glut-7 contains an extra 6 amino acid (KKMKND) at the C-terminal end. This sequence is very similar to the last 6 amino acids of some of the microsomal UDP-glucoronosyltransferase isoenzymes (see refs. 1). The KK-K-- has been considered motif for the retention of transmembrane proteins and is not found in Gluts.
Applications
- Western blot (1 - 10 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in ELISA and Western Blot. Western Blot:1-10 ug/ml using ECL. Detects a major band at about 52kD in various tissues. ELISA: 0.5-1 ug/ml, Control peptide can be used to coat ELISA plates at 1 ug/ml.
Presentation
100 mM Tris, pH 7.4, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee