Human RBX1 / ROC1

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Delipidated and defibrinated followed by sterile filtration

Unmodified

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 94-108 of Human ROC1 (C-terminal) coupled to KLH.

aa94-108

Reacts with human, mouse, C.elgans and zebra fish ROC1. Cross reactivity may also occur with ROC1 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC2 and APC11.

• IHC
• IHC - Paraffin (1:500)
• Western blot (1:500 - 1:1000)
• ELISA (1:2000 - 1:10000)

Immunohistochemistry: LS-B415 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B415 was determined to be 1:500.

Antiserum, 0.01% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P62877 NCBI: NM_014248 NP_055063.1