Target
Mouse Peripherin
Synonyms
PRPH | NEF4 | Neurofilament 4 (57kD) | Peripherin | PRPH1 | Neurofilament 4
Reactivity
Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-QKEQHSDLDKSSIH, from the C-Terminus of protein sequence according to NP_038667.2NP_001157060.1NP_001157061.1.
Specificity
Mouse Peripherin. This antibody is expected to recognize all reported isoforms (NP_038667.2; NP_001157060.1; NP_001157061.1).
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 55kD band observed in Rat Colon lysates (calculated MW of 54.0kD according to NP_036765.2). Recommended concentration: 0.1-0.3 ug/ml. An additional faint band of unknown identity was also consistently observed at 75kD. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee