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Catalog Number Size Price
LS-C482569-50 50 µl (1 mg/ml) $286 
LS-C482569-100 100 µl (1 mg/ml) $321 
LS-C482569-200 200 µl (1 mg/ml) $386 
NOL3 / ARC Antibody - Immunohistochemical analysis of Nop30 staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NOL3 / ARC Antibody - Immunofluorescent analysis of Nop30 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
NOL3 / ARC Antibody - Western blot analysis of Nop30 expression in BT474 (A); MCF7 (B); mouse heart (C); mouse lung (D); rat heart (E) whole cell lysates.
NOL3 / ARC Antibody - Immunohistochemical analysis of Nop30 staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NOL3 / ARC Antibody - Immunofluorescent analysis of Nop30 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
NOL3 / ARC Antibody - Western blot analysis of Nop30 expression in BT474 (A); MCF7 (B); mouse heart (C); mouse lung (D); rat heart (E) whole cell lysates.
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Polyclonal Rabbit anti‑Human NOL3 / ARC Antibody (IHC, IF, WB) LS‑C482569

Polyclonal Rabbit anti‑Human NOL3 / ARC Antibody (IHC, IF, WB) LS‑C482569

Antibody:
NOL3 / ARC Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$286
LS-C482569-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
NOL3 / ARC Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ARC antibody LS-C482569 is an unconjugated rabbit polyclonal antibody to ARC (NOL3) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human NOL3 / ARC
Synonyms
NOL3 | Apoptosis repressor with CARD | CARD2 | FCM | NOP | Nucleolar protein 3 | Nucleolar protein of 30 kDa | MYP | NOP30
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Recombinant full length human Nop30.
Specificity
Recognizes endogenous levels of Nop30 protein.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:200)
  • ICC (1:10 - 1:100)
  • Immunofluorescence (1:10 - 1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOL3 / ARC
O60936 NM_003946 NP_003937.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/23/2024