Target
Human KLRK1 / CD314 / NKG2D
Synonyms
KLRK1 | D12S2489E | CD314 antigen | KLR | NK cell receptor D | NKG2D | NKG2-D | NKG2-D-activating NK receptor | CD314
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-KVYSKEDQDLLK, from the internal region of the protein sequence according to NP_031386.2.
Specificity
Human KLRK1 / NKG2D.
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 35 kDa band observed in human lymph node and human spleen lysates (calculated MW of 25.3 kDa according to NP_031386.1). The observed molecular weight is explained by glycosylation (Han et al, Blood. 2004 Nov 1;104(9):2858-66. Epub 2004 Jul 6; PMID: 15238421). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KLRK1 / CD314 / NKG2D