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Antibodies > ITIH4 > LS-C757839
 
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Catalog Number Size Price
LS-C757839-200 200 µl $393 
ITIH4 Antibody - Anti-ITIH4 Antibody (C-Term) at 1:2000 dilution + human plasma lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 103 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
ITIH4 Antibody - Overlay histogram showing U-2OS cells stained with ITIH4 Antibody (C-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (ITIH4 Antibody (C-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
ITIH4 Antibody - Anti-ITIH4 Antibody (C-Term) at 1:2000 dilution + human plasma lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 103 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
ITIH4 Antibody - Overlay histogram showing U-2OS cells stained with ITIH4 Antibody (C-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (ITIH4 Antibody (C-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Polyclonal Rabbit anti‑Human ITIH4 Antibody (aa885‑917, WB) LS‑C757839

Polyclonal Rabbit anti‑Human ITIH4 Antibody (aa885‑917, WB) LS‑C757839

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Antibody:
ITIH4 Rabbit anti-Human Polyclonal (aa885-917) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757839-200
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ITIH4 Rabbit anti-Human Polyclonal (aa885-917) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ITIH4 antibody LS-C757839 is an unconjugated rabbit polyclonal antibody to human ITIH4 (aa885-917). Validated for ELISA, Flow and WB.
Target
Human ITIH4
Synonyms
ITIH4 | gp120 | H4P | ITIHL1 | PK120 | ITI heavy chain H4 | PK-120 | IHRP | ITI-HC4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Ig Polyclonal
Conjugations
Unconjugated
Purification
Protein A and Peptide Affinity Chromatography
Modifications
Unmodified
Immunogen
This ITIH4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 885-917 amino acids from human ITIH4.
Epitope
aa885-917
Applications
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • ELISA
Usage
Western Blot Predicted Molecular Weight: 103.4 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ITIH4
Q14624 NM_002218 NP_002209.2

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ITIH4 Antibody - Western Blot;Sample: Recombinant ITIH4, Human
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ITIH4 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling ITIH4 with ITIH4 Antibody (C-Term) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).
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ITIH4 ELISA Kit
Human ITIH4 (Sandwich ELISA) ELISA Kit - LS-F6535
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Range: 0.39-25 ng/ml
See all ITIH4 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/30/2024
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United States

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