Human ITGAV/Integrin Alpha V/CD51

Rabbit

Human, Mouse, Goat, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Antiserum

Unmodified

Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha V integrin subunit.

C-Terminus

Recognizes CD51 (Integrin alpha V). The epitope recognized is on the cytoplasmic domain of the protein and it is thus intracellular. No cross-reactivity to alpha 1, alpha 2, alpha 3, alpha 4 or alpha 6 integrin subunits. Species cross-reactivity: Human, goat, equine, porcine, sheep and mouse.

• IHC
• IHC - Frozen (1:500)
• Western blot (1:250)
• Immunoprecipitation
• ELISA (1:500)
• Radioimmunoassay (1:500)

Suitable for use in ELISA, RIA, Western Blot, Immunoprecipitation and Immunohistochemistry. ELISA: 1:500. RIA: 1:500. Western Blot: 1:250. Recognizes a single band of 150kD when denatured, but not reduced. Under reducing conditions recognizes two major bands of 25 and 27kD, and to a lesser extent the full length 150kD band. This pattern is consistent with the fact that mature alpha V is composed of a disulfide bonded heavy (125kD) and a light (25kD) chains derived from a proteolytically cleaved precursor. Immunoprecipitation: 10 ul of antibody is sufficient to precipitate alpha V/beta 1 from 5x1E6 cells. Immunohistochemistry: 1:500. Acetone fixed tissue only.

0.5% sodium azide, before the addition of glycerol to 40%

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P06756 NCBI: NM_002210 NP_002201.1